Loh Jia Tong’s Lab


Although it is well-recognized that our immune system matures and evolves from infancy to adulthood as posited by the hygiene hypothesis, the underlying phenotypic and functional differences between the pediatric and adult immune system, and how they can be shaped by pathogenic triggers and dietary factors, remain poorly understood. This has resulted in limited, non-specific, and inefficacious treatment options available for this group of vulnerable individuals, leaving them at risk of suffering from permanent damage, long-term complications, decreased quality of life, and even death.

As such, the objective of our lab is to uncover the unknowns of immunoregulation and immunopathogenesis in children during health and diseases using preclinical mouse models. This could aid in the (1) the identification of novel and specific therapeutic targets in children, (2) identification of children who are at risk of developing severe complications associated with infectious diseases for early intervention, and (3) development of novel vaccine adjuvants capable of promoting a more robust and prolonged immunity in children to reduce the need for booster shots.

Loh Jia Tong
Assistant Professor

Email: [email protected]
Irene Soon Ai Ling
Project Officer

Email:  [email protected]


  • Elucidating Dok3-calprotectin axis in neutrophils to identify potential therapeutic targets for management of severe COVID-19
  • Understanding immune responses and regulation in children towards the development of therapeutics and vaccine adjuvants for pediatric diseases
  • Investigating the effects of high sugar diet during weaning on immune development and imprinting 
  • Loh JT*, Teo JKH, Kannan S, Verma C, Andiappan A, Lim HH, Lam KP*. DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit signaling mediator CARD11. Science Signaling. (Manuscript accepted) (*Co-corresponding author)
  • Loh JT, Teo JKH, Kannan S, Verma C, Lim HH, Lam KP. Disrupting Dok3-Card9 interaction with synthetic peptides enhances antifungal effector functions of human neutrophils. Pharmaceutics. (2023). Doi: 10.3390/pharmaceutics15071780
  • Teo KYW, Zhang S, Loh JT, Lai RC, Hey HWD, Lam KP, Lim SK, Toh WS. Mesenchymal stromal cell exosomes mediate M2-like macrophage polarization through CD73/ecto-5’-nucleotidase activity. Pharmaceutics. (2023) doi: 10.3390/pharmaceutics15051489
  • Loh JT* and Lam KP*. Fungal infections: Immune defense, immunotherapies and vaccines. Adv. Drug Deliv. Rev. (2023). doi: 10.1016/j.addr.2023.114775 (*Co-corresponding author)
  • Loh JT and Lam KP. Neutrophils in the pathogenesis of rheumatic diseases. Rheumatol Immunol Res. (2022). doi: 10.2478/rir-2022-0020
  • Loh JT*, Teo JKH, Lam KP*. Dok3 restrains neutrophil production of calprotectin during TLR4 sensing of SARS-CoV-2 spike protein. Front. Immunol. (2022). doi: 10.3389/fimmu.2022.996637 (*Co-corresponding author)
  • Loh JT, Zhang B, Teo JKH, Lai RC, Choo ABH, Lam KP, Lim SK. Mechanism for the attenuation of neutrophil and complement hyperactivity by MSC exosomes. Cytotherapy. (2022) S1465-3249(22)00018-4.doi:10.1016/j.jcyt.2021.12.003.
  • Loh JT, Lee KG, Lee AP, Teo JKH, Lim HL, Kim SSY, Tan AHM, Lam KP. DOK3 maintains intestinal homeostasis by suppressing JAK2/STAT3 signaling and S100a8/9 production in neutrophils. Cell Death Dis. 12, 1054 (2021). https://doi.org/10.1038/s41419-021-04357-5
  • Wang X, Lin X, Zheng Z, Lu B, Wang J, Tan AHM, Zhao M, Loh JT, Ng SW, Chen Q, Xiao F, Huang En, Ko K, Huang Z, Li J, Kok K, Lu G, Liu X, Lam KP, Liu W, Zhang Y, Yuen K, Mak TW, Lu L. Host-derived lipids orchestrate pulmonary gd T cell response to provide early protection against influenza virus infection. Nat. Commun. 12, 1914(2021). Https://doi.org/10.1038/s41467-021-22242-9
  • Loh JT*, Teo JKH, Lim HH and Lam KP*. Emerging roles of Downstream of Kinase 3 in cell signaling. Front. Immunol. (2020) 11:566192. doi: 10.3389/fimmu.2020.566192 (*Co-corresponding author)
  • Loh JT, Xu S, Huo JX, Kim SS, Wang Y, Lam KP. Dok3-protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity. J Clin Investigat. (2019) 129:2717-29. doi: 10.1172/jci126341
  • Loh JT, TJF Lim, K Ikumi, T Matoba, B Janela, M Gunawan, T Toyama, M Bunjamin, LG Ng, M Poidinger, A Morita, F Ginhoux, S Yamazaki, KP Lam, IH Su. Ezh2 controls skin tolerance through distinct mechanisms in different subsets of skin dendritic cells. iScience. (2018) 10:23-39. doi: 10.1016/j.isci.2018.11.019
  • Chung HH, Or YZ, Shrestha S, Loh JT, Lim CL, Ong Z, Woo ARE, Su IH, Lin VCL. Estrogen reprograms the activity of neutrophils to foster protumoral microenvironment during mammary involution. Sci Rep. (2017) 7:46485. doi: 10.1038/srep46485
  • Loh JT and Su IH. Post-translational modification-regulated leukocyte adhesion and migration. Oncotarget. (2016) 7(24): 37347-37360. doi: 10.18632/oncotarget.8135
  • Loh JT, Gunawan M, Su IH. Ear inflammation and whole-mount ear staining. Bio-protocol. (2016) 6(20):e1967.
  • Gunawan M*, Venkatesan N*, Loh JT*, Wong JF, Berger H, Neo WH, Li LY, La Win MK, Yau YH, Guo T, See PCE, Yamazaki S, Chin KC, Gingras A, Shochat SG, Ng LG, Sze SK, Ginhoux F, Su IH. The methyltransferase Ezh2 controls cell adhesion and migration through direct methylation of the extranuclear regulatory protein talin. Nat. Immunol. (2015) 16:505-516. doi: 10.1038/ni.312 (*co-first author)