Wu Bin’s lab

Group Photo

​DESCRIPTION OF RESEARCH WORK

  1. Structural characterization of filamentous protein complexes using helical reconstruction of cryo-EM images. Death Domain oligomers, protein/nucleic acid oligomers, etc.
  2. Structure based development of novel biotechnological tools.
  3. Engineer novel protein/nucleic acid signaling complexes to manipulate relevant biological processes.
Wu BinLead​ PI
Wu Bin
Associate Professor

Email: wubin@ntu.edu.sg
Phone: (65) 6908 2207
Office: EMB-06-06
Chong Han Chung, KelvinChong Han Chung Kelvin
Research Fellow

Email: kelvin.hcchong@ntu.edu.sg
Chai Yoke Tin
Project Officer

Email: ytchai@ntu.edu.sg
Rae ChuaRae Chua
Co-supervised PhD student

Email: RAEC0002@e.ntu.edu.sg
Tan Yong Jun, EddieTan Yong Jun Eddie
PhD student

Email: TANY0517@e.ntu.edu.sg
Xu Chen RuiXu Chenrui
PhD student

Email: xuch0013@e.ntu.edu.sg
Huang Fu Zhi RuHuangfu Zhiru
Master student

Email: ZHIRU001@e.ntu.edu.sg

CURRENT PROJECTS

  1. Non-disclosed industrial project 1, related to protein engineering
  2. Non-disclosed industrial project 2, related to innate immunity
  3. MOH-000382: Determining the molecular and structural basis for inflammasome activation in human
PUBLICATIONS
  1. M Huang, X Zhang, GA Toh, Q Gong, J Wang, Z Han, B Wu, L Zhong, and J Chai Structural and biochemical mechanisms of NLRP1 inhibition by DPP9. Nature, (2021) Mar 17; 
    https://doi.org/10.1038/s41586-021-03320-w
  2. Q Gong§, R Kim§, C Xu, PT Huynh, H Chong, YJ Tan, J Zhang, ZZ Boo, Teo ET, K Lay, Y Zhang, J Lim, WI Goh, G Wright, L Zhong, B Reversade, B Wu Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8. Nature Commun. (2021) Jan 8;
    https://doi.org/10.1038/s41467-020-20319-5
  3. A Stsiapanava§, C Xu§, M Brunati, S Zamora-Caballero, C Schaeffer, L Han, M Carroni, S Yasumasu, L Rampoldi, B Wu# and L Jovine# Cryo-EM Structure of Native Human Uromodulin, a Zona Pellucida Module Polymer. EMBO J (2020) Dec 15; 39:e106807;
    https://doi.org/10.15252/embj.2020106807
  4. YS Huoh, B Wu, S Park, D Yang, K Bansal, E Greenwald, WP Wong, D Mathis, S Hur Dual functions of Aire CARD multimerization in the transcriptional regulation of T cell tolerance. Nature Commun. (2020) Apr 2; 11, Article number: 1625;
    https://doi.org/10.1038/s41467-020-15448-w
  5. Y Deng, T Wu, M Wang, S Shi, G Yuan, X Li, H Chong, B Wu, P Zheng Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level. Nature Commun.  (2019) Jun 24;10(1):2775;
    https://doi.org/10.1038/s41467-019-10696-x
  6. Q Gong, Z Long, L Zhong, ET Teo, Y Jin, Z Yin, ZZ Boo, Y Zhang, J Zhang, R Yang, S Bhushan, B Reversade, Z Li, B Wu Structural Basis of RIP2 Activation and Signaling. Nature Commun.  (2018) Nov 26;9(1):4993;
    https://doi.org/10.1038/s41467-018-07447-9
  7. R Yang, YH Wong, GKT Nguyen, JP Tam, J Lescar#, B Wu# Engineering a catalytically efficient recombinant protein ligase Journal of the American Chemical Society 139 (15), 5351-5358 (Cover Article of 2017 April 19th issue, Research highlight of the year);
    https://doi.org/10.1021/jacs.6b12637
  8. B Wu, A Peisley, D Tetrault, Z Li, EH Egelman, KE Magor, T Walz, P Penczek, S Hur Molecular imprinting as a signal-activation mechanism of the viral RNA sensor RIG-I (2014) Molecular cell 55 (4), 511-523;
    https://doi.org/10.1016/j.molcel.2014.06.010
  9. A Peisley, B Wu, X Hui, ZJ Chen and S Hur Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Nature (2014) May 1; 509:110-114. PMID: 24590070;
    https://doi.org/10.1038/nature13140
  10. B Wu, A Peisley, C Richards, H Yao, X Zeng, C Lin, F Chu, T Walz, S Hur Structural Basis for dsRNA recognition, filament formation and antiviral signaling by MDA5. Cell 2013 Jan 17;152(1-2):276-89.  PMID: 23273991;
    https://doi.org/10.1016/j.cell.2012.11.048