CURRENT PROJECTS

1. Non-disclosed industrial project 1, related to protein engineering
2. Non-disclosed industrial project 2, related to innate immunity
3. MOH-000382: Determining the molecular and structural basis for inflammasome activation in human
4. MOH-001216 Determining the molecular basis of Noddosome activation in human

PUBLICATIONS

1. Z Ku, X Xie, J Lin, P Gao, B Wu, AEI Sahili, H Su, Y Liu, X Ye, EYJ Tan, X Li, X Fan, BC Goh, W Xiong, H Boyd, AE Muruato, H Deng, H Xia, J Zou, BK Kalveram, VD Menachery, N Zhang, J Lescar^, PY Shi^, Z An^ Engineering SARS-CoV-2 specific cocktail antibodies into a bispecific format improves neutralizing potency and breadth. Nature communications 13 (1), 5552 (2022/9/22)
   
2. Joey Kuok Hoong Yam, Thet Tun Aung, Song Lin Chua, Yingying Cheng, Gurjeet Singh Kohli, Jianuan Zhou, Florentin Constancias, Yang Liu, Zhao Cai, May Margarette Santillan Salido, Daniela I Drautz-Moses, Scott A Rice, Stephan Christoph Schuster, Zhao Zhi Boo, Bin Wu, Staffan Kjelleberg, Tim Tolker-Nielsen, Rajamani Lakshminarayanan, Roger W Beuerman, Liang Yang, Michael Givskov Elevated c-di-GMP levels and expression of the type III secretion system promote corneal infection by Pseudomonas aeruginosa. Infection and immunity 90 (8), e00061-22 (2022/8/18)
   
3. Ricardo Moreno Traspas, Tze Shin Teoh, Pui-Mun Wong, Michael Maier, Crystal Y Chia, Kenneth Lay, Nur Ain Ali, Austin Larson, Fuad Al Mutairi, Nouriya Abbas Al-Sannaa, Eissa Ali Faqeih, Majid Alfadhel, Huma Arshad Cheema, Juliette Dupont, Stéphane Bézieau, Bertrand Isidor, Dorrain Yanwen Low, Yulan Wang, Grace Tan, Poh San Lai, Hugues Piloquet, Madeleine Joubert, Hulya Kayserili, Kimberly A Kripps, Shareef A Nahas, Eric P Wartchow, Mikako Warren, Gandham SriLakshmi Bhavani, Majed Dasouki, Renata Sandoval, Elisa Carvalho, Luiza Ramos, Gilda Porta, B Wu, Harsha Prasada Lashkari, Badr AlSaleem, Raeda M BaAbbad, Anabela Natália Abreu Ferrão, Vasiliki Karageorgou, Natalia Ordonez-Herrera, Suliman Khan, Peter Bauer, Benjamin Cogne, Aida M Bertoli-Avella, Marie Vincent, Katta Mohan Girisha, Bruno Reversade^ Loss of FOCAD, operating via the SKI messenger RNA surveillance pathway, causes a pediatric syndrome with liver cirrhosis. Nature genetics 54 (8), 1214-1226 Published: 21 July 2022
   
4. KS Robinson, GA Toh, P Rozario, R Chua*, S Bauernfried, Z Sun, MJ Firdaus, S Bayat, R Nadkarni, ZS Poh, KC Tham, CR Harapas, CK Lim, W Chu, CWS Tay, KY Tan, T Zhao, C Bonnard, R Sobota, JE Connolly, J Common, SL Masters, KW Chen, Lena Ho, B Wu, V Hornung^, FL Zhong^ ZAKα-driven ribotoxic stress response activates the human NLRP1 inflammasome. Science 14 Jul 2022 Vol 377, Issue 6603, pp. 328-335
   
5. D Yu§, W Song§, EYJ Tan§*, L Liu, Y Cao, J Jirschitzka, E Li, E Logemann, C Xu*, S Huang, A Jia, X Chang, Z Han, B Wu^, P Schulze-Lefert^, J Chai^ TIR domains of plant immune receptors are 2′, 3′-cAMP/cGMP synthetases mediating cell death. Cell Accepted 26 April 2022, Available online 20 May 2022, Version of Record 23 June 2022.
   
6. A Stsiapanava, C Xu*, S Nishio, L Han, N Yamakawa, M Carroni, K Tunyasuvunakool, J Jumper, D Sanctis, B Wu, L Jovine Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH. Nature Structural & Molecular Biology (2022) 29, pages 190–193.
   
7. B Song, Y Chen, X Liu, F Yuan, EYJ Tan*, Y Lei, N Song, Y Han, BD Pascal, PR Griffin, C Luo, B Wu, D Luo, J Zheng Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains. Immunity (2021) 54 (10), 2218-2230. e5 
8. M Huang, X Zhang, GA Toh, Q Gong, J Wang, Z Han, B Wu, L Zhong, and J Chai Structural and biochemical mechanisms of NLRP1 inhibition by DPP9. Nature, (2021) Mar 17; 
   https://doi.org/10.1038/s41586-021-03320-w
9. Q Gong§, R Kim§, C Xu, PT Huynh, H Chong, YJ Tan, J Zhang, ZZ Boo, Teo ET, K Lay, Y Zhang, J Lim, WI Goh, G Wright, L Zhong, B Reversade, B Wu Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8. Nature Commun. (2021) Jan 8;
   https://doi.org/10.1038/s41467-020-20319-5
10. A Stsiapanava§, C Xu§, M Brunati, S Zamora-Caballero, C Schaeffer, L Han, M Carroni, S Yasumasu, L Rampoldi, B Wu# and L Jovine# Cryo-EM Structure of Native Human Uromodulin, a Zona Pellucida Module Polymer. EMBO J (2020) Dec 15; 39:e106807;
    https://doi.org/10.15252/embj.2020106807
11. YS Huoh, B Wu, S Park, D Yang, K Bansal, E Greenwald, WP Wong, D Mathis, S Hur Dual functions of Aire CARD multimerization in the transcriptional regulation of T cell tolerance. Nature Commun. (2020) Apr 2; 11, Article number: 1625;
    https://doi.org/10.1038/s41467-020-15448-w
12. Y Deng, T Wu, M Wang, S Shi, G Yuan, X Li, H Chong, B Wu, P Zheng Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level. Nature Commun.  (2019) Jun 24;10(1):2775;
    https://doi.org/10.1038/s41467-019-10696-x
13. Q Gong, Z Long, L Zhong, ET Teo, Y Jin, Z Yin, ZZ Boo, Y Zhang, J Zhang, R Yang, S Bhushan, B Reversade, Z Li, B Wu Structural Basis of RIP2 Activation and Signaling. Nature Commun.  (2018) Nov 26;9(1):4993;
    https://doi.org/10.1038/s41467-018-07447-9
14. R Yang, YH Wong, GKT Nguyen, JP Tam, J Lescar#, B Wu# Engineering a catalytically efficient recombinant protein ligase Journal of the American Chemical Society 139 (15), 5351-5358 (Cover Article of 2017 April 19^th issue, Research highlight of the year);
    https://doi.org/10.1021/jacs.6b12637
15. B Wu, A Peisley, D Tetrault, Z Li, EH Egelman, KE Magor, T Walz, P Penczek, S Hur Molecular imprinting as a signal-activation mechanism of the viral RNA sensor RIG-I (2014) Molecular cell 55 (4), 511-523;
    https://doi.org/10.1016/j.molcel.2014.06.010 16
16. A Peisley, B Wu, X Hui, ZJ Chen and S Hur Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Nature (2014) May 1; 509:110-114. PMID: 24590070;
    https://doi.org/10.1038/nature13140
17. B Wu, A Peisley, C Richards, H Yao, X Zeng, C Lin, F Chu, T Walz, S Hur Structural Basis for dsRNA recognition, filament formation and antiviral signaling by MDA5. Cell 2013 Jan 17;152(1-2):276-89.  PMID: 23273991;
    https://doi.org/10.1016/j.cell.2012.11.048