Wu Bin’s lab
DESCRIPTION OF RESEARCH WORK
- Structural characterization of filamentous protein complexes using helical reconstruction of cryo-EM images. Death Domain oligomers, protein/nucleic acid oligomers, etc.
- Structure based development of novel biotechnological tools.
- Engineer novel protein/nucleic acid signaling complexes to manipulate relevant biological processes.
![]() | Lead PI
Wu Bin Associate Professor Email: [email protected] Phone: (65) 6908 2207 Office: EMB-06-06 |
![]() | Chong Han Chung Kelvin
Research Fellow Email: [email protected] |
![]() | Chai Yoke Tin
Project Officer Email: [email protected] |
![]() | Rae Chua
Co-supervised PhD student Email: [email protected] |
![]() | Tan Yong Jun Eddie
PhD student Email: [email protected] |
![]() | Xu Chenrui
PhD student Email: [email protected] |
![]() | Huangfu Zhiru
Master student Email: [email protected] |
CURRENT PROJECTS
- Non-disclosed industrial project 1, related to protein engineering
- Non-disclosed industrial project 2, related to innate immunity
- MOH-000382: Determining the molecular and structural basis for inflammasome activation in human
PUBLICATIONS
- M Huang, X Zhang, GA Toh, Q Gong, J Wang, Z Han, B Wu, L Zhong, and J Chai Structural and biochemical mechanisms of NLRP1 inhibition by DPP9. Nature, (2021)
Mar 17;
https://doi.org/10.1038/s41586-021-03320-w - Q Gong§, R Kim§, C Xu, PT Huynh, H Chong, YJ Tan, J Zhang, ZZ Boo, Teo ET, K Lay, Y Zhang, J Lim, WI Goh, G Wright, L Zhong, B Reversade, B Wu Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8. Nature Commun. (2021) Jan 8;
https://doi.org/10.1038/s41467-020-20319-5 - A Stsiapanava§, C Xu§, M Brunati, S Zamora-Caballero, C Schaeffer, L Han, M Carroni, S Yasumasu, L Rampoldi, B Wu# and L Jovine# Cryo-EM Structure of Native Human Uromodulin, a Zona Pellucida Module Polymer. EMBO J (2020) Dec 15; 39:e106807;
https://doi.org/10.15252/embj.2020106807 - YS Huoh, B Wu, S Park, D Yang, K Bansal, E Greenwald, WP Wong, D Mathis, S Hur Dual functions of Aire CARD multimerization in the transcriptional regulation of T cell tolerance.
Nature Commun. (2020) Apr 2; 11, Article number: 1625;
https://doi.org/10.1038/s41467-020-15448-w - Y Deng, T Wu, M Wang, S Shi, G Yuan, X Li, H Chong, B Wu, P Zheng Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level. Nature Commun. (2019) Jun 24;10(1):2775;
https://doi.org/10.1038/s41467-019-10696-x - Q Gong, Z Long, L Zhong, ET Teo, Y Jin, Z Yin, ZZ Boo, Y Zhang, J Zhang, R Yang, S Bhushan, B Reversade, Z Li, B Wu Structural Basis of RIP2 Activation and Signaling. Nature Commun. (2018) Nov 26;9(1):4993;
https://doi.org/10.1038/s41467-018-07447-9 - R Yang, YH Wong, GKT Nguyen, JP Tam, J Lescar#, B Wu# Engineering a catalytically efficient recombinant protein ligase Journal of the American Chemical Society 139
(15), 5351-5358 (Cover Article of 2017 April 19th issue, Research highlight of the year);
https://doi.org/10.1021/jacs.6b12637 - B Wu, A Peisley, D Tetrault, Z Li, EH Egelman, KE Magor, T Walz, P Penczek, S Hur Molecular imprinting as a signal-activation mechanism of the viral RNA sensor RIG-I (2014) Molecular cell 55 (4), 511-523;
https://doi.org/10.1016/j.molcel.2014.06.010 - A Peisley, B Wu, X Hui, ZJ Chen and S Hur Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Nature (2014) May 1; 509:110-114. PMID: 24590070;
https://doi.org/10.1038/nature13140 - B Wu, A Peisley, C Richards, H Yao, X Zeng, C Lin, F Chu, T Walz, S Hur Structural Basis for dsRNA recognition, filament formation and antiviral signaling by MDA5. Cell 2013 Jan 17;152(1-2):276-89. PMID: 23273991;
https://doi.org/10.1016/j.cell.2012.11.048