• Discovery of Druggable Biologics from Medicinal Plants
• Large-scale Conversion of Wastes to Functional Food and High-value Products
• Discovery, Engineering and Applications of Peptide Ligases
• Mode-of-Action of Druggable Biologics from Medicinal Plants
• Mechanisms of Active ligases

Full list of publications can be found here

• Hemu, X., Zhang, X., Chang, H. Y., Poh, J. E., & Tam, J. P. (2023). Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation. Journal of Biological Chemistry, 299(3). https://doi.org/10.1016/j.jbc.2023.102997  
  
• Xia, Y., Li, F., Zhang, X., Balamkundu, S., Tang, F., Hu, S., Lescar, J., Tam, J. P., & Liu, C.-F. (2023). A Cascade Enzymatic Reaction Scheme for Irreversible Transpeptidative Protein Ligation. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.2c13628 
  
• Julca, I., Mutwil-Anderwald, D., Manoj, V., Khan, Z., Lai, S. K., Yang, L. K., Beh, I. T., Dziekan, J., Lim, Y. P., Lim, S. K., Low, Y. W., Lam, Y. I., Tjia, S., Mu, Y., Tan, Q. W., Nuc, P., Choo, L. M., Khew, G., Loo, S., Kam, A., Tam, J.P., Bozdech, Z., Schmidt, M., Usadel, B., Kanagasundaram, Y., Alseekh, S., Fernie, A., Li, H. Y., Mutwil, M. (2023). Genomic, transcriptomic, and metabolomic analysis of Oldenlandia corymbosa reveals the biosynthesis and mode of action of anti-cancer metabolites. Journal of Integrative Plant Biology. https://doi.org/10.1111/jipb.13469 
  
• Hemu, X., Chan, N.-Y., Liew, H.T., Hu, S., Zhang, X., Serra, A., Lescar, J., Liu, C.-F. and Tam, J.P. (2023), Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains. New Phytol. https://doi.org/10.1111/nph.18841 
  
• Dutta B., Loo S., Kam A., Sze SK., Tam J.P. (2023) Ginsentide TP1 Protects Hypoxia-Induced Dysfunction and ER Stress-Linked Apoptosis. Cells. https://doi.org/10.3390/cells12101401 
  
• Tam, J.P., Huang, J., Loo, S., Li, Y., & Kam, A. (2023). Ginsentide-Like Coffeetides Isolated from Coffee Waste Are Cell-Penetrating and Metal-Binding Microproteins. Molecules. https://doi.org/10.3390/molecules28186556  
  
• To, J., Zhang, X., Tam, J.P. (2023). Design of Potent and Salt-Insensitive Antimicrobial Branched Peptides. Polymers. https://doi.org/10.3390/polym15173594 
  
• Dutta B., Loo S., Kam A., Tam J. P. (2023). Plant-derived cell-penetrating microprotein α-astratide aM1 targets Akt signaling and alleviates insulin resistance. Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-023-04937-y 
• Vascular Bed Molecular Profiling by Differential Systemic Decellularization In Vivo
• One-pot dual labeling of an IgG 1 and preparation of C-to-C fusion proteins through a combination of Sortase A and Butelase 1
• Ginsentides: Cysteine and Glycine-rich Peptides from the Ginseng Family with Unusual Disulfide Connectivity
• Plant-derived Mitochondria-targeting Cysteine-rich Peptide Modulates Cellular Bioenergetics
• Astratides: insulin-modulating, insecticidal and antifungal cysteine-rich peptides from Astragalus membranaceus
• Ligase-controlled Cyclo-oligomerization of Peptides
• Pulsed SILAC-based proteomic analysis unveils hypoxia- and serum starvation-induced de novo protein synthesis with PHD finger protein 14 (PHF14) as a hypoxia sensitive epigenetic regulator in cell cycle progression
• Hypoxia-induced tumor exosomes promote M2-like macrophage polarization of infiltrating myeloid cells and microRNA-mediated metabolic shift
• Immobilization and Intracellular Delivery of Circular Proteins by Modifying a Genetically Incorporated Unnatural Amino Acid
• Molecular diversity and function of jasmintides from Jasminum sambac​