Inverted Fluorescence Microscope - TiE

Nikon Ti-E Motorised Inverted Microscope with PFS; manual XY Stage

• Transmitted light Imaging, long-working distance condenser N.A. 0.52 W.D. 30 mm, phase contrast optics

• Widefield fluorescence Imaging using XCite 120LED lamp

• DAPI, GFP, mCherry, Cy5 filter sets

• Objective Lenses: CFI Plan Fluor 4x/0.13, 10x/0.30, ELWD 20x/0.45, ELWD 40x/0.60, ELWD 60x/0.70

• Orca Flash 4.0 V2 sCMOS camera, Nikon colour camera

• MetaMorph software for image acquisition and analysis

Brightfield and phase-contrast imaging on live cells, fixed cells; fluorescence imaging on live cells, fixed cells