TOWARDS UNDERSTANDING AND APPLICATIONS OF ANTISENSE OLIGONUCLEOTIDES

ORAL DEFENCE

Antisense oligonucleotides (ASOs), which are synthetic single-stranded nucleic acids, could enter living cells, hybridize to messenger RNAs and inhibit gene expression. This approach could potentially lead to desired alterations of cellular characteristics, and has been under intensive investigations. This thesis aimed to build a substitute model towards the molecular mechanisms of ASO, and develop some therapeutic ASOs. The effects of various cellular and molecular conditions to an ASO efficacy are modelled by mathematical equations and practical experimentations. Particularly, three aspects are investigated separately, namely (i) the competition of productive and non-productive ASO uptakes, (ii) the ASO sequestration by intracellular proteins, (iii) the extent of mRNA expression. The unknown amount of functional ASOs delivered into the cells was also estimated. These findings enhance our understanding on antisense oligonucleotide therapeutics, potentially contributing to the discovery of more advanced ASO generations in the future. Finally, ASOs against 2 gene targets of interest responsible for autoimmune disease and cancer, respectively, have been developed, showing promising therapeutic outcomes.

Supervisor: Prof Phan Anh Tuan