Non-targeted Screening of Bioactive Chemicals in Water by Nuclear Receptor Protein Affinity Selection - Mass Spectrometry (23 Nov)

While exposure to environmental endocrine disrupting chemicals (EDCs) can cause extensive health issues, the specific EDCs in environment remain elusive. A non-target analysis based on nuclear receptor protein affinity selection - mass spectrometry was developed and used to high-throughout identification of ERα, LXRα, PPARγ, RAR, or RXR bioactive chemicals in drinking water and the river water in Changjinag and Huanghe river basins. The antiretroviral drug efavirenz was for the first time identified to be LXR antagonistic chemical which was also identified to induce foam cell formation through LXRα antagonism, demonstrating efavirenz-related atherosclerotic effects.