Molecular Ecology Laboratory

The laboratory is equipped for molecular biology analyses (e.g., DNA extraction from environmental samples, sequencing library preparation) and microscopic observation and documentation.

 

Equipment: DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field

Model/Brand: BluePippin

Specifications

  • Electrophoresis voltage: 25V, 100V or 150V constant, 100V pulsed field
  • Optical detection: 470 nm excitation, 525 nm emission

Applications

DNA capabilities of the Pippin Prep and pulsed-field electrophoresis for resolving and collecting high molecular weight DNA

 

 

 

Equipment: NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer

Model/Brand: ND-ONE-W Thermo Scientific

Specifications

  • Accuracy (Photometric): 3% (at 0.97 A)
  • Wavelength Accuracy: ±1 nm
  • Wavelength Range: 190–850 nm
  • dsDNA: Pedestal: 27,500 ng/µL
  • BSA (IgG): Pedestal: 820 (400) mg/mL
  • Pathlength (Metric): 0.030 to 1.0 mm auto-ranging
  • Photometric Range: 10 mm equivalent; Pedestal: 0–550 A Cuvette: 0–1.5 A
  • Measurement Repeatability: Typical: 0.002 A (1.0 mm path) or 1%CV, whichever is greater (SD of 10 individual measurements at 0.97 A)

Applications

For improved measurement accuracy and contaminant identification. Quantify and qualify DNA, RNA, and protein samples.

 

 

Equipment: Nexus Gradient MasterCycler Thermal Cycler

Model/Brand: 6331 Eppendorf

Specifications

  • Sample Capacity: 96 x 0.1 ml PCR tubes, 96 x 0.2ml PCR tubes, 71 x 0.5ml PCR tubes, 1 x 96-well PCR plate
  • Thermoblock temperature range: 4°C - 99°C
  • Ramp rate: Max. 3°C/s
  • Thermoblock temperature homogeneity: At 35°C: ≤ 3°C; At 90°C: ≤ 4°C
  • Control accuracy: ± 0.2°C

Applications

For PCR applications

 

Equipment: Labcycler Gradient

Model/Brand: Sensoquest

Specifications

  • Heated Lid: Programable: Pressure 0 to 120 N, temperature RT up to 110 °C
  • Gradient: 40 °C, ± 20 °C from left to right
  • Temperature: -5.0 °C to 99.9 °C
  • Ramp rate: 0.001 °C – 5.0 °C
  • Homogeneity: ± 0.25 °C @ 55 °C, ± 0.4 °C @ 95 °C
  • De(In)crements Time: ± 99.99 s, temperature: ± 9.99 °C

Applications

For classic PCR with temperature gradients from 8 to 24 zones to determine the optimal annealing temperature


Equipment: Centrifuge

Model/Brand: 5810R Eppendorf

Specifications

  • Temperature range: -9 °C to 40 °C
  • Speed: 200 – 14,000 rpm
  • Max. capacity: 4 × 750 mL/4 × 4 MTP
  • Rotors available: 18
  • Acceleration/braking ramps: 10/10
  • Cooling: Refrigerated

Applications

Separate or concentrate substances suspended in a liquid medium by density

 

Equipment: Microscope

Model/Brand: CKX53 Olympus

Specifications

  • Observation Method: Fluorescence (Blue/Green Excitation)
  • Observation Tubes: Widefield (FN 22) Trinocular
  • Illuminator : Transmitted Illuminator LED Lamp
  • Condenser:NA 0.3/ W.D. 72 mm (built in)

Applications

For a variety of cell culture needs, including live cell observation, cell sampling and handling, image capture, and fluorescence observation.

 

 

Equipment: Microscope

Model/Brand: BX-53 Olympus

Specifications

CCD camera:
  • Olympus XM10 monochrome camera
  • 1.4 Mpixel CCD with 1376x1032 pixels (6.45 mm pixel size).

  • Live frame rate from 15fps at 1376x1032 to 80 fps at 172x129

Olympus DP26 color camera:
  • 5 Mpixel CCD with 2448 x 1920 pixels (3.45 mm pixel size)
  • Live frame rate from 7 fps at full frame to 31 fps at 320x480

Applications

Equipped with monochrome and color cameras provides excellent fluorescence and color images.