LONZA 4D- NUCLEOFACTOR CORE, X AND Y UNIT

The Stem Cell Core Platform functions as a multi-user shared research facility dedicated to support and accelerate studies involving human stem cells at LKCMedicine, in the industry and beyond.

The Platform can help maintain and provide well-characterised human embryonic stem cell (hESC) lines and human-induced pluripotent stem cell (iPSC) lines in addition to the training in essential culture techniques for human pluripotent stem cells. For further enquiries, please contact For enquiries, please contact Dr Ng Sean Pin at seanngsp@ntu.edu.sg or Ken Wong @ kenwongkn@ntu.edu.sg 

In summary, the services provide the following:

• HiPSC line generation using nonintegrating methods, with further characterisation

• Two-day hands-on class on the basic methods of culturing human embryonic and pluripotent stem cells (hESC and HiPSC)

• Distribution of hESC and hiPSC from LKCMedicine stem-cell bank

• Use of the facility to culture and expand customised hESC and iPSCs

• Consultation and support for internal and external stem cell projects

• Gene-targeting in iPSCs using the CRISPR/Cas9 to generate single gene knockouts

Automated cell counters

• Real-time cell analysers

• Cell storage freezers and tanks

• Fully equipped tissue culture facilities with BSCs, CO2 incubators, waterbath, centrifuges, fridges, microscopes, etc

The controlling unit of the 4D-Nucleofector^TM System is suited to handle various functional units for efficient transfection of hard-to-transfect cell lines and primary cells in different formats.