Microscope

High-modality TIRF Microscope - NSTORM

HIGH MODALITY TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF) MICROSCOPE

CSB L11 Support Room 7
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Inverted TIRF microscope with 405nm, 445nm, 488nm, 561nm and 647nm laser lines

Objective lenses: 10x/0.3 Plan Fluor, 20x/0.5 Plan Fluor, 60x/1.49 oil Plan Apo TIRF and 100x/1.49 oil Plan Apo TIRF

Motorised stage, filter turret, nosepiece

Stage-top incubation chamber with temperature control

Hardware autofocus for longterm Imaging

High quantum efficiency (QE~95%) sCMOS camera for TIRF applications, and a second sCMOS camera for high-speed applications such as STORM imaging

FRAP capability

Suitable for imaging events occurring near cell membrane in live/fixed cells, XYZ-t imaging, FRAP, Tile and multi-position imaging. Depending of the structure of interest and labelling chemistry resolution down to 20-40 nm can be achieved