The NISB Cryo-Electron Microscopy Platform at NTU for imaging biological samples was set up to support the research community in NTU and Singapore. The applications include Single Particle Analysis (SPA) of purified proteins and viruses, Cryo-Electron Tomography (Cryo-ET) of cell sections, bacterial and tissue samples, Micro Electron Diffraction (MicroED), Cryo-Focused Ion Beam (Cryo-FIB) of creating a compression-free ultra-thin cryo lamella samples for TEM tomography imaging, and Correlative Light Electron Microscope (CLEM) to image proteins inside the cell, and in isolations, using novel labeling techniques.
Tecnai-12 (T12) iCorr - 120kV TEM
An entry level 120 keV electron microscope and capable for correlated light and electron microscopy studies
Tecnai Arctica - 200kV Cryo-TEM
A 200 keV cryo-EM equipped with direct electron detector and volta phase plate is capable to achieve 3 Å resolution, screen 12 samples in one go, providing an opportunity to boost the contrast in biological samples.
Titan Krios - 300kV Cryo-TEM
High-end 300 keV cryo-EM capable to achieve highest sub-2 Å resolution, load 12 sample at a time and capable to collect 200-500 times more images in a day with direct electron detectors
Aquilos Cryo-FIB - Cryo-DualBeam System
A cryo focused ion beam (dual beam) scanning EM capable to prepare up to 8-10 compression-free ultrathin lamella in a day and identify biological samples using a light microscope, which subsequent used for electron microscopic analysis.
CorrSight - Correlative Light and Electron Microscope
Corrsight, a correlative light and electron microscope (CLEM), is build for live microscopy experiment and observation of vitrified samples
Safety and facility access
User safety is one of our top priorities. As such, new users who wish to access our facilities must read through our Cryo-EM Lab Safety Rules.
Users who wish to use our instruments are required to read through our Platform Guidelines and Rules and prepare the relevant documents.
Please submit all completed documents to firstname.lastname@example.org.
Users who wish to utilize our instruments are required to book them before use. To book our instruments, please click here.
Do read through our document for EM laboratory good practices before using the equipment.
To view our charges please click here.
For queries regarding equipment utilization, training or other matters, please contact any of our platform managers.