Assoc. Prof. Pervushin, Konstantin
Associate Professor
Phone: (65) 6791 1744
Email: [email protected]
Our lab focuses on advancing solution-state NMR spectroscopy to investigate the structure and dynamics of biologically relevant protein systems. We use and develop state-of-the-art NMR techniques—such as TROSY, CRINEPT, LTROSY, and TROSY-SPI—to study medium to large proteins and protein complexes, including refolded and membrane proteins, multimeric enzymes, ion channels, and molecular machines involved in heme transfer and protein folding. A key biological target is IAPP and its analogues, whose conformational dynamics we probe using tailored NMR relaxation methods. We also work on NMR hardware, pulse sequence, and computational developments. Our contributions include a novel TROSY concept enabling structural studies of large complexes (~220 kDa) and the creation of SideLink, a tool for automatic resonance assignment and direct spectrum analysis, significantly accelerating protein structure determination.
Research Areas
Solution NMR Spectroscopy of Biomolecules, NMR Method Development, Protein Dynamics and Folding, Large Protein Complexes, Structural studies of amyloids
Research Fellow
PhD Student
Nivedita_Kumar
Research Associate
- Lim J, Chin S, Miserez A, Xue K, Pervushin K. Trifluoroacetic acid as a molecular probe for the dense phase in liquid–liquid phase-separating peptide systems. Anal Chem. 2025;97(1):166–174. https://doi.org/10.1021/acs.analchem.4c03444
- Torres J, Pervushin K, Surya W. Prediction of conformational states in a coronavirus channel using Alphafold-2 and DeepMSA2: Strengths and limitations. Comput Struct Biotechnol J. 2024;23:3730–3740. https://doi.org/10.1016/j.csbj.2024.10.021
- Lim J, Gudlur S, Buchanan C, Perrin QM, Boyd H, Moulin M, Iwase H, Porcar P, Cárdenas M, Miserez A, Pervushin K. Hierarchical structural organization in bioinspired peptide coacervate microdroplets. bioRxiv. 2024;1(1). https://doi.org/10.1101/2024.07.06.602323
- Low JYK, Shi X, Anandalakshmi V, Neo D, Peh GSL, Koh SK, Zhou L, Abdul Rahim MK, Boo K, Lee J, Mohanram H, Alag R, Mu Y, Mehta JS, Pervushin K. Release of frustration drives corneal amyloid disaggregation by brain chaperone. Commun Biol. 2023;6(1):348. https://doi.org/10.1038/s42003-023-04725-1
- Li M, Mirshafian R, Wang J, Mohanram H, Ahn KA, Hosseinzadeh S, Pervushin KV, Waite JH, Yu J. Compliant clients: Catechols exhibit enhanced solubility and stability in diverse complex coacervates. Biomacromolecules. 2023;24(9):4190–4198. https://doi.org/10.1021/acs.biomac.3c00519