Flow Cytometry

Flow Cytometry has the ability to discriminate, quantify and sort distinct population of cells based on expression of markers with rapid ease thus opening up new research opportunities or aid in current research Work. A summary of how flow cytometry is used is found here.

 

CONTACT INFORMATION

Location of FacilitySchool of Biological Sciences
02S-68 / 02S-69
(Flow Cytometry Room)
Contact Details​Professor Christiane Ruedl
(Scientific Consultant and person in charge)
Email Address: [email protected]
Dr Abdul Rashid B M Muzaki
(FACS Consultant and Training)
Email Address: [email protected]

BD FACS Aria 1 Sorter and BD FACS Aria 3 Sorter

SPECIFICATIONS
  • Multicolor analysis for up to 7 parameters.
  • 2 air-cooled lasers at 488-nm Blue, 633-nm Red.
  • Digital acquisition rates of up to 70,000 events/second.
  • Two- and four-way bulk sorting devices for a variety of tube sizes.

ADDITIONAL OPTION
  • Automated Cell Deposition Unit (ACDU) for sorting to multi-well plates and microscopic slides.

APPLICATION
  • For non-sterile sorting of bacteria, yeast and algae.
  • Direct sort (single-cells or bulk) into PCR tubes for RNA preparation work.
  • High-speed cell sorting and single cell sorting into 96 well plate.

BD FACS Aria 1 Sorter and BD FACS Aria 3 Sorter

SPECIFICATIONS
  • Multicolor analysis for up to 15 parameters
  • 4 air-cooled lasers at 355-nm UV, 488-nm Blue, 561-nm Yellow/Green and 633-nm Red
    BD FACS Aria 3 Sorter laser types
  • Digital acquisition rates of up to 70,000 events/second
  • Two- and four-way bulk sorting devices for a variety of tube sizes

ADDITIONAL OPTION
  • Automated Cell Deposition Unit (ACDU) for sorting to 96 multi-well plates and microscopic slides.

APPLICATION
  • Only for sterile sorting of mammalian cells and liposomes.
  • Direct sort (single-cells or bulk) into PCR tubes for RNA preparation work.
  • High-speed cell sorting and single cell sorting into 96-well plate.

 

 

BD 3-Lasers Fortessa X20 with High-Throughput Sampler (HTS)

SPECIFICATIONS
  • Multicolor analysis for up to 10 parameters
  • 3 air-cooled lasers at 355-nm UV, 488-nm Blue and 633-nm Red
  • Digital acquisition rates of up to 70,000 events/second
  • HTS option

Fortessa X-20 (3-laser) configuration

BD 5-Lasers Fortessa X20

SPECIFICATIONS
  • Multicolor analysis for up to 18 parameters.
  • 5 air-cooled lasers at 355-nm UV, 405-nm Violet, 488-nm Blue, 561-nm Yellow/Green and 633-nm Red.
  • Digital acquisition rates of up to 70,000 events/second.

Fortessa X-20 (5-laser) configuration

The Symphony A5.2 Full-spectral 50-channel flow-cytometer is equipped with the latest cutting-edge technology in flow-cytometry. It uses a novel technology enabling sample acquisition in both full-spectral and conventional modes. This unique feature is not present in our other flow-cytometers. The advantage of acquisition in full-spectral mode is that users can now distinguish closely similar dyes apart which often remains a problem in our current flow-cytometers. In addition, since spectral spill-over of signals into adjacent channels contributing to noisy resolution is a major issue in our current conventional flow-cytometer, this new full-spectral feature eliminates this problem. Students and researchers can now be accustomed to this new full-spectral technology which is set to become the future in flow-cytometry. Aligning with NTU’s mission to be at the forefront of education and research, this new equipment shall pave the way to new discoveries by removing the hurdles in fluorochrome panel design.

SPECIFICATIONS
  • Simultaneous analysis for up to 50 parameters.
  • Full-spectral acquisition capable.
  • 5 air-cooled lasers at 355-nm UV, 405-nm Violet, 488-nm Blue, 532-nm Yellow/Green and 628-nm Red.
  • Digital acquisition rates of up to 70,000 events/second.
  • Attached High-Throughput Sampler

CHARGES FOR USE OF EQUIPMENT

EQUIPMENT $/h for SBS,
(Usage with HTS)
$/h for SPMS, (Usage with HTS) $/h LKC, (Usage with HTS)
FortessaX20 3-laser 15 (30) 30 (50) 40 (60)
FortessaX20 5-laser 15 (30) 30 (50) 40 (60)
BD FACSymphonyA5.2 15 (30) 50 (70) 60 (80)
FacsAria 50 80 100
Training charge 50 50 50
Service (Run Samples) 100 150 200


EQUIPMENT
$/h for CoS, (Usage with HTS) $/h for non-NTU users
Fortessa X20 3-laser 40 (60) 80 (110)
Fortessa X20 5-laser 40 (​60) 80 (110)
BD FACSymphonyA5.2 60 (80) 120 (150)
FACSAria 100 300
Training charge 50 100
Service (Run Samples) 200 400

Procedures for use of equipment

  • Internal Users are to use the Online Facilities Booking System to book their usage at least a day in advance
  • New and inexperienced users are to contact the Dr Rashid to attend a 2 hour training before they are allowed to use the flow-cytometers
  • Users are encouraged to email the contact in-charge for discussion of experiments and use of equipment
​​​​​​​​​