Research Capabilities

State of the art facilities encompassing of the following areas:

Microscopy Facility

Confocal laser scanning fluorescence microscopy
Light Sheet fluorescence microscopy
Live cell fluorescence imaging
Total Internal Reflection Fluorescence (TIRF) imaging
Epifluorescence stereomicroscope imaging

Mass Spectrometer

MALDI-TOF/TOF
LC-MS/MS
4-plex iTRAQ
6-plex and 10-plex tandem mass tags (TMT)
Whole proteome identification
Phosphopeptide enrichment and analysis
N-glycopeptide enrichment and analysis
SILAC metabolic labelling system

Flow Cytometry

High throughput analysis of up to 5 colours
High throughput cell sorting

Biomolecular Interaction Platform

Biacore Surface Plasmon Resonance system
Isothermal titration microcalorimeter
Dynamic Light Scattering system
Circular dichroic spectrometer
Analytical ultracentrifuge
Fluorescence spectrophotometer with fluorescence, phosphorescence, chemiluminescence, and bioluminescence capabilities

Protein Production Platform

High-throughput cloning and testing of protein expression constructs, with Escherichia coli as the main workhorse
Mammalian and insect cell systems for more difficult target proteins
Advice and suggestions for designing expression constructs
Liter-scale cultures and medium-throughput protein purification using FPLC systems
Project management and data sharing on a laboratory information management system

Nuclear Magnetic Resonance

Solution state NMR ranging from 400 to 700 MHz
Biomolecular structural, interaction, and functional studies