DawnHeleos II-Optilab T-rEX - SEC-MALS

The HPLC system with UV and fluorescence detector uses size exclusion chromatography (SEC) to separate samples based on their retention volume. The eluted peaks can be analyzed with DawnHeleos-II Multi-Angle Light Scattering (MALS) detector and Optilab T-rEX refractive index (RI) detector.

If you are interested in using the equipment or require assistance and training, please kindly contact us
SEC-MALS, size exclusion chromatography used in conjunction with Multi-Angle Light Scattering (MALS) analysis, is a technique allows accurate measurement of sample's molecular weight and hydrodynamic radius/radius of gyration in solution. Our system allows characterization of polymers (heterogenic and synthetic), protein oligomers and aggregates, and conjugates.

Shimadzu HPLC Prominence: 

  • UV-VIS and fluorescence detector
  • Column oven
  • Autosampler
  • Sample volume: < 100uL
  • SEC columns (S200 10/300 Increase and S200 5/150GL)

 

DawnHeleos-II & Optilab T-rEX:

  • 18 angles light scattering detector
  • Comet (built-in ultrasonic device)
  • Interferometric refractometer