DESCRIPTION OF LAB RESEARCH WORK
The main interest of my lab is to understand the biology of animal and human RNA viruses. In particular we wish to understand, at the molecular level, the role that host-cell factors play during specific stages of the virus replication cycle, such as virus replication and assembly. It is hoped that a detailed understanding of the intimate relationship between these individual viruses and the host-cell during infection will lead to the development of novel antiviral strategies. The current interests include (a) Paramyxoviruses (RSV, hMPV, hNV): To understand the role that host factors play during virus assembly. (b) Human and avian influenza virus: The focus is on the interaction between the polymerase complex and host factors that determine species adaptation (c) The development of a vaccine against EMCV. This research uses a multidisciplinary experimental approach which employs molecular biology, imaging technology, functional genomics and proteomics.
- Host factors that play a role in RSV and HMPV assembly
- Biological properties of human and avian influenza virus polymerase complex.
- Developing a vaccine against EMCV infection
- A System Based-Approach to Examine Cytokine Response in Poxvirus-Infected Macrophages. (2018) Wong PS, Sutejo R, Chen H, Ng SH, Sugrue RJ, Tan BH. Viruses. 10(12).
- Comparative Transcriptomic and Metagenomic Analyses of Influenza Virus-Infected Nasal Epithelial Cells From Multiple Individuals Reveal Specific Nasal-Initiated Signatures. (2018). Tan KS, Yan Y, Koh WLH, Li L, Choi H, Tran T, Sugrue R, Wang Y, Chow VT. Front Microbiol. 2018 9:2685.
- A sustained antiviral host response in respiratory syncytial virus infected human nasal epithelium does not prevent progeny virus production. (2018) Huong TN, Yan Y, Jumat MR, Lui J, Tan BH, Wang Y, Sugrue RJ. Virology. 521:20-32. (Cover page)
- Taye B, Yeo D, Lee RTC, Tan BH, Sugrue RJ*, Maurer-Stroh S*. (2017) Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains. Int J Mol Sci. 18(11). (*co-corresponding).
- Taye B, Vaz C, Tanavde V, Kuznetsov VA, Eisenhaber F, Sugrue RJ, Maurer-Stroh S. (2017) Benchmarking selected computational gene network growing tools in context of virus-host interactions. Sci Rep. 19;7(1):5805.
- Taye B, Chen H, Myaing MZ, Tan BH, Maurer-Stroh S, Sugrue RJ. (2017) Systems-based approach to examine the cytokine responses in primary mouse lung macrophages infected with low pathogenic avian Influenza virus circulating in South East Asia. BMC Genomics. 30;18(1):420.
- Ludwig A, Nguyen TH, Leong D, Ravi LI, Huan TB, Sandin S, Sugrue RJ. (2017) Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. J Cell Sci. 130(6):1037-1050. (Article highlighted).
- Myaing M, Jumat R, Huong T, Tan BH, Sugrue RJ. (2017) Truncated forms of the PA protein containing only the C-terminal domains are associated with the RNP complex within H1N1 influenza virus particles. J Gen Virol. 98: 906-921
- Tra Nguyen Huong, Boon Huan Tan, and Richard J. Sugrue. (2016). A proteomic workflow using purified respiratory syncytial virus particles to identify cellular factors as drug targets. RSV drug targeting. in Methods in Molecular Biology series. Humana Press. 1442, 175-194.
- Yan Y, Tan KS, Li C, Tran T, Chao SS, Sugrue RJ, Shi L, Chow VT, Wang DY.(2016) Human nasal epithelial cells derived from multiple subjects exhibit differential responses to H3N2 influenza virus infection in vitro. J Allergy Clin Immunol. 138(1):276-281.e15