Published on 24 Nov 2020

Strategies to Slow the Development of Daptomycin Resistance in Enterococcus faecalis

SBS-IAS Seminar by Associate Prof Kimberly Kline

Modification of anionic lipids by multiple peptide resistance factor (MprF) renders E. faecalis more resistant to killing by defensins and less susceptible to focal targeting by the cationic antimicrobial peptides. MprF mutants are both more sensitive to killing by daptomycin and evolve daptomycin resistance more slowly. Understanding the mechanism by which MprF mutants slow antimicrobial resistance acquisition has enabled the proposal of new combinatorial strategies to prolong the efficacy of this last line antibiotic.