Welcome to Sanyal Lab

 

The 3D Chromatin Architecture, Transcription and Genomics (3D CATG) laboratory is located at School of Biological Sciences, Nanyang Technological University, Singapore. The laboratory investigates how 3D structure and organization of genome influence cell- and tissue- specific gene expression in development, differentiation and disease conditions. Sanyal Lab employs high-throughput genomics, genome-editing and imaging techniques in combination with bioinformatics and computational approaches to understand structure-function relationship of chromatin. The ultimate goal is to decipher systems-level understanding of complex relationships between genome architecture, gene transcription, chromatin and epigenetic features (DNase I hypersensitivity, histone modifications, DNA methylation, etc.) and binding of transcription factors in normal development and disease.

The broad research themes of the laboratory are:

  • Generation of high-resolution chromatin interaction maps of interphase nuclei using Chromosome Conformation Capture (3C)-based tools and imaging techniques to study genome architecture
  • Studying long-range looping interactions between gene promoters and distal cis-regulatory elements in normal and pathological conditions
  • Understanding the role of non-coding GWAS variants (SNPs) and identification of their target genes in complex diseases like cancer, neurological and metabolic disorders, etc.
  • Systems-level modelling of structure-function relationship of chromatin using bioinformatics and computational approaches
  • Experimental perturbation of chromatin architecture using genome engineering and editing tools for functional characterization and validation of looping interactions

Functional organization of the genome

 

In our lab, we intend using high-throughput genomic methods, genome-editing and imaging techniques in combination with bioinformatics and computational approaches to understand structure-function relationship of chromatin.

Keywords: 3D genome organization, Chromatin, Chromosome Conformation Capture (3C), Cis-regulatory elements, Enhancer, Gene Promoter, Transcription, Epigenetics, Genome-wide association studies (GWAS), Single nucleotide polymorphism (SNP), Complex disease, Genome editing